Library Pooling Calculator

Desired library pool molarity (nM)

Desired library pool volume (µL)

Number of libraries

Sample Name

PCR type

Conc (ng/µL)

Avg. length (bp)

Desired Molarity (nM)

Sample vol. to pool (µL)

Expected sequencing library pool concentration: 0.000 ng/µL 0.000 fmol/ul

Water to be added to the sequencing library pool: 0 µL

Linear dsDNA Libraries

Pool A library

Sample Name

PCR type

Conc (ng/µL)

Avg. length (bp)

Desired Molarity (nM)

Sample vol. to pool (µL)

Pool B library

Sample Name

PCR type

Conc (ng/µL)

Avg. length (bp)

Desired Molarity (nM)

Sample vol. to pool (µL)

Pool A concentration: 0.000 ng/µL

Pool B concentration: 0.000 ng/µL

Water to Pool A: 0.00 µL Water to Pool B: 0.00 µL

Circular ssDNA Libraries

Targeted sequencing library pool input (fmol). (Check with your sequencing provider.)

Sample Name

Conc (ng/µL)

Avg. length (bp)

Sample vol. to pool (µL)

circular ssDNA Pool A

circular ssDNA Pool B

Expected sequencing library pool concentration: 0.000 fmol/ul

After pooling, Qubit ssDNA measurement

Sample Name

Conc (ng/µL)

Avg. length (bp)

fmol/ul

Sequencing library pool