RNA BaseCode Technology

Same Gene, Different Jobs

Proteins produced from the same gene can perform dramatically different functions.

Distinguishing between RNA isoforms is essential to fully understand the diverse roles that genes and their products play in both health and disease.

Diagram illustrating the process of pre-mRNA splicing, showing exons and introns, spliceosome components, and the removal of introns with the formation of mature mRNA.

Short-read sequencing misses the message

Short-read sequencing is the gold standard for high-throughput gene expression analysis and offers unmatched accuracy, scalability, and availability, enabling precise quantification of gene expression across thousands of samples. While short reads may cover exon junctions, they fail to provide a complete and accurate view and distinguish RNA isoforms.

Diagram showing how proteins synthesize RNA from DNA, illustrating transcription with molecules labeled and color-coded for different stages.

RNA BaseCode Synthetic long-reads fill in the gaps

The RNA BaseCode chemistry uniquely barcodes all short-reads that originate from the same RNA molecule. By creating a random pattern of errors during reverse-transcription, all cDNA originating from a single RNA molecule can be identified. These short reads are then combined to create synthetic long-read data of exceptional quality and accuracy.

Diagram showing cDNA synthesis to introduce a base code pattern, featuring T24 PCR handle, short-read sequencing library with short reads, and RNA base code synthetic long-read data.
Infographic with six icons and accompanying text explaining RNA sequencing technology. The icons include a circle with lines representing RNA, a DNA strand with multiple layers, a test tube rack with sample tubes, a circular arrow with a globe, a gear with a globe, and horizontal lines.

The RNA BaseCode Library Preparation protocol is an easy-to-follow workflow that is clear, fast, and accessible and does not need any complex equipment or infrastructure.

Our proprietary library preparation chemistry is optimized to maximize full-length cDNA generation and minimize any biases towards shorter fragments that are common for other RNA sequencing library preparation methods.

Flowchart illustrating DNA sequencing process, including RNA isolation, library preparation, sequencing, and data analysis with visual icons for lab tools and computer.
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