RNA BaseCode Technology

Person wearing green gloves holding a box labeled RNA BaseCode Sequencing Library Kit with samples inside

Same Gene, Different Jobs

Proteins produced from the same gene can perform dramatically different functions.

Distinguishing between RNA isoforms is essential to fully understand the diverse roles that genes and their products play in both health and disease.

Diagram illustrating the process of pre-mRNA splicing, showing exons and introns, spliceosome components, and the removal of introns with the formation of mature mRNA.

Short-read sequencing misses the message

Short-read sequencing is the gold standard for high-throughput gene expression analysis, offering unmatched accuracy, scalability, and accessibility. It enables precise quantification of gene expression across thousands of samples. However, while short reads can capture exon–exon junctions, they cannot fully resolve or accurately distinguish RNA isoforms.

Diagram showing how proteins synthesize RNA from DNA, illustrating transcription with molecules labeled and color-coded for different stages.

RNA BaseCode Synthetic long-reads fill in the gaps

The RNA BaseCode Technology unlocks a complete view of the transcriptome by transforming short-read data into long-read output.

Our chemistry uniquely barcodes each cDNA molecule during reverse-transcription.

This barcode identifies all the short-reads originated from the same RNA molecule, that can then be combined into synthetic long-read data of exceptional quality and accuracy.

Diagram showing cDNA synthesis to introduce a base code pattern, featuring T24 PCR handle, short-read sequencing library with short reads, and RNA base code synthetic long-read data.

Infographic with six icons and accompanying text explaining RNA sequencing technology. The icons include a circle with lines representing RNA, a DNA strand with multiple layers, a test tube rack with sample tubes, a circular arrow with a globe, a gear with a globe, and horizontal lines.

Key Features and Benefits of RNA BaseCode

RNA BaseCode Library Preparation - How it works

The RNA BaseCode Library Preparation protocol offers an easy, streamlined workflow that’s clear, fast, and accessible, without requiring complex equipment or specialized infrastructure.vOur proprietary chemistry is specifically optimized to maximize full-length cDNA generation while minimizing the bias toward shorter fragments often seen with traditional RNA sequencing library preparation methods.

Flowchart illustrating DNA sequencing process, including RNA isolation, library preparation, sequencing, and data analysis with visual icons for lab tools and computer.

Learn More about the RNA BaseCode Kit and Service.